TruCytes™ Potency: Scalable Cell Mimics for Reproducible CAR Assays

A Scalable, Reproducible Alternative to Cell Line Targets for CAR-Based Potency Assays

Optimize Functional CAR-T Potency Readouts

These lyophilized, customizable cell mimics express clinically relevant antigens (e.g., CD19, CD20, BCMA) at controlled densities—enabling clear and consistent cytokine release without the hurdles of cell-line engineering, maintenance, or chain-of-custody.

Key Advantages of TruCytes Potency

Limited Supply: TruCytes™ Potency CD20 Evaluation Kit

Our TryCytes Potency CD20 Evaluation kit helps CAR-T developers assess functional potency using Slingshot’s precision-engineered cell mimics. Each kit supports assay development and optimization with targeted activation, consistent cytokine release, and full technical support. Order now to secure early access and take advantage of introductory pricing.

Component	Description
TruCytes Potency CD20 + Enhancer	High-activation cell mimic
TruCytes Potency Negative	Non-activating negative cell mimic
Technical Support	Full FAS support and expert consultation

The TruCytes™ Potency CD20 Evaluation Kit is part of an early release program intended for research use only. Performance data is based on internal studies and early adopter feedback. Kit availability is limited, and specifications are subject to refinement as additional validation is completed. Not for use in diagnostic procedures.

TruCytes™ Potency CD19 Evaluation Kit

Our TryCytes Potency CD19 Evaluation kit helps CAR-T developers assess functional potency using Slingshot’s precision-engineered cell mimics. Each kit supports assay development and optimization with targeted activation, consistent cytokine release, and full technical support.

Component	Description
TruCytes Potency CD19 + Enhancer	High-activation cell mimic
TruCytes Potency CD19	Baseline activation cell mimic
TruCytes Potency Negative	Non-activating negative cell mimic
Technical Support	Full FAS support and expert consultation

The TruCytes™ Potency CD19 Evaluation Kit is part of an early release program intended for research use only. Performance data is based on internal studies and early adopter feedback. Kit availability is limited, and specifications are subject to refinement as additional validation is completed. Not for use in diagnostic procedures.

TruCytes™ Potency BCMA Evaluation Kit

Our TryCytes Potency BCMA Evaluation kit helps CAR-T developers assess functional potency using Slingshot’s precision-engineered cell mimics. Each kit supports assay development and optimization with targeted activation, consistent cytokine release, and full technical support.

Component	Description
TruCytes Potency BCMA + Enhancer	High-activation cell mimic
TruCytes Potency BCMA	Baseline activation cell mimic
TruCytes Potency Negative	Non-activating negative cell mimic
Technical Support	Full FAS support and expert consultation

The TruCytes™ Potency BCMA Evaluation Kit is part of an early release program intended for research use only. Performance data is based on internal studies and early adopter feedback. Kit availability is limited, and specifications are subject to refinement as additional validation is completed. Not for use in diagnostic procedures.

AVAILABLE NOW

Application Note

Following ISCT 2025 presentation, our full application note is now available. It details how TruCytes Potency cell mimics were evaluated compared with tumor cell lines. Data included demonstrates strong, sustained IFN-γ release, reduced nonspecific activation, and superior reproducibility.

Get your free copy below.

Where TruCytes Potency Makes a Difference

Assay Development

Tunable antigen density allows developers to modulate activation strength refining assay sensitivity

Quality Control

Precision-engineered manufacturing delivers consistent activation profiles across runs, sites, and operators

Tech Transfer

Consistent performance helps establish functional assays at new facilities with fewer bridging studies or delays

Download Our Free TruCytes™ Potency Application Note

Share a few details about your assay and antigen of interest. Our team will follow up to coordinate an evaluation kit or discuss a custom TruCytes Potency solution tailored to your workflow. Let’s accelerate your potency assay development.

Understanding the Mechanism of Action for TruCytes Potency

TruCytes™ Potency mimics are designed to provide a highly controlled environment for evaluating CAR-T cell functionality. By expressing clinically relevant antigens at precise densities, these cell mimics facilitate a clear understanding of how CAR-T cells interact with target antigens, allowing researchers to assess the mechanism of action (MoA) effectively.

This understanding is crucial for optimizing CAR-T therapies, as it enables developers to fine-tune their approaches based on specific immune responses. With consistent cytokine release and reduced variability, researchers can better predict the therapeutic potential of their CAR-T products in clinical settings.

Comparative Analysis: TruCytes vs. Traditional Cell Lines

When evaluating potency assays, researchers often face the choice between using traditional cell lines and innovative solutions like TruCytes™ Potency. Unlike traditional cell lines that can exhibit significant variability and require extensive maintenance, TruCytes offer a standardized, reproducible alternative that simplifies assay development.

This comparative analysis highlights the advantages of TruCytes, including their ability to provide consistent results across different experiments and operators. The reduced complexity of using TruCytes not only accelerates the qualification process but also enhances the overall reliability of potency testing in CAR-T therapies.

Real-World Applications of TruCytes Potency in CAR-T Development

TruCytes™ Potency has been successfully implemented in various CAR-T development projects, showcasing its versatility and effectiveness. Researchers have utilized these cell mimics to streamline their potency assays, leading to more rapid development timelines and improved product consistency.

Case studies illustrate how companies have leveraged TruCytes to enhance their assay methodologies, resulting in significant improvements in product performance and regulatory compliance. These real-world applications underscore the transformative potential of TruCytes in advancing CAR-T cell therapies.

Ordering and Support for TruCytes Potency Products

Ordering TruCytes™ Potency products is straightforward, with options available for various evaluation kits tailored to specific antigens. Customers can easily navigate the online platform to select the appropriate kit for their research needs, ensuring they receive the right tools for accurate potency assessments.

Additionally, Slingshot Biosciences provides robust technical support for all TruCytes products, helping researchers optimize their assays and troubleshoot any challenges. This commitment to customer support ensures that users can maximize the benefits of using TruCytes in their CAR-T development processes.