Calibration Made Easier
Flow cytometry is a critical cellular analysis tool used across immunology, diagnostics, cancer research, and cell therapy. The speed and sensitivity of the technology also leads to lots of headaches to ensure accurate and consistent performance. Add to this complexity is the reality that instrument performance varies day-to-day and instrument-to-instrument.
Therefore, instrument standardization and calibration are essential to achieve accurate, reproducible, and comparable results. Yet the methods to do so are painstaking and time consuming. The primary reason is that the tools to calibrate cytometry instruments have historically been biological specimens or latex beads, both of which have major drawbacks.
There is now a better solution: synthetic cells. This first-of-its-kind technology combines the accuracy of real biological cells coupled with the consistency of synthetic production. Learn more below about how synthetic cells are enabling instrument calibration and standardization to be done easier!
Calibration is complex
No two cytometers are exactly alike. With many components in fluidics, optics, and sensors, each instrument is unique. Yet all these parts must work in perfect symphony to achieve precision.
Moreover, high parameter cytometry assays are now the norm, which requires greater sensitivity and precision. Thus calibration is a complex task as instruments grow in sophistication.
Roadblocks to better calibration
Current tools to calibrate instrument performance are limited to biospecimens and latex beads.
Biospecimens suffer from short shelf-life, high lot-to-lot variability, transport restrictions, and biohazard handling. This makes the calibration process sub-optimal.
In contrast, latex beads cannot cannot match the light scattering properties of real cells, which is a critical feature in cell analysis. Also, they exhibit high autofluorescence with many dyes, especially in the UV and violet range. The result is an inaccurate surrogate of cells.
Precise surrogate controls
The ideal solution is to combine both: realistic cell features with the reproducibility of synthetic materials.
FlowCytes are the revolutionary cell surrogate controls that combines the best of both. Replicate the scatter accuracy of real cells with the benefit of long shelf-life, low variability, and no biohazard handling.
Additional breakthroughs include low autofluorescence, accurate spectral signatures, and surface coating of biomarkers.
Achieve newfound performance
Eliminate time spent qualifying QC material, resolving inaccurate data, and salvaging unclear results. Maximize data quality with more accurate calibration.
Synthetic cell controls enable production teams, sales, field engineers, and application scientists to quickly standardize instrument performance. The result is a more accurate, precise, and consistent instrument.